A central
composite experimental design was used to evaluate optimal conditions of the
medium component, pH and temperature for cyclodextrin glycosyltransferase
production from sorghum by Bacillus circulans (American Type Culture
Collection).
Sorghum
concentration, initial pH and temperature were optimized using the
response surface method. Maximal CGTase production of 216.94 U.mL-1 was
obtained after 48 hours of fermentation when the concentrations of the medium
were 0.16% sifted sorghum, 0.5% triptone, 0.5% yeast extract, 1% sodium
carbonate, 0.1% K2HPO4, 0.02% MgSO4•7 H2O, initial pH of 8.0 and temperature of
35°C under rotational shaking.
Further studies
aimed at increasing the fermentation scale using optimal conditions for the
high enzymatic production and high volumetric productivity are needed to
improve the fermentation process described in the present study.
