Listeria
monocytogenes is afoodborne pathogen with significant impacts on public health
and economy worldwide. Reliable and fast detection of L. monocytogenes is of
major importance for both diagnostic laboratories and the food industry.
The current study
evaluated the performance of the Assurance GDS® assay for the detection of L.
monocytogenes in pure culturesand spiked food samples. In the pure culture
experiments, the Assurance GDS® assay for Listeria
monocytogenesaccurately detected the target strains of different
serotypesand was correctly negative for a variety of other Listeria species.
For reliable
detection of L. monocytogenes in pure culture experiments, colony counts
>105 cfu/ml were required, which emphasizes the need for an adequate
enrichment step.
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