Rice is one of the most important
cereal food crops in the world and Indica-type rice provides the staple food
for more than half of the world’s population. In spite of its large scale
production, a number of abiotic and biotic factors have limited its
productivity. To meet the growing demands of an ever-increasing global
population, genetic transformation of Indica rice, particularly for public
breeding materials, has become a matter of increased urgency.
Many factors are known to affect
the efficiency of T-DNA delivery to plant cells, such as different plant
varieties, different explant types, the quality of explants and Agrobacterium
strains [6], the cell density of Agrobacterium
for infection, inoculation period, co-culture medium and desiccation stress
of the tissue before or after infection.Indica rice is still known as
recalcitrant for transformation although Japonica rice was transformed using
the Agrobacteriummediated method since 1994 and Indica rice in 1996. Few
reports on the Agrobacterium-mediated transformation of IR58025B are available.
This public cultivar is commonly
applied to generate hybrids by breeders in the south Asia since it has higher
combining ability and generates good hybrids. Therefore, there is a need to
evaluate various factors that affect tissue culture and to develop a highly
efficient Agrobacterium-mediated transformation protocol for this public
cultivar. Hiei et al.Reported a protocol for Group I Indica rice
transformation including IR58025B using immature embryos as explants. Our study
mainly focused on callus explants derived from mature seeds and systematically
optimized several factors that could influence transformation frequency.
No comments:
Post a Comment